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Autocrine growth mechanism by transforming growth factor (TGF)‐β 1 and TGF‐β 1 ‐receptor regulation by epidermal growth factor in a human endometrial cancer cell line IK‐90
Author(s) -
Sakata Masahiro,
Kurachi Hirohisa,
Ikegami Hiromasa,
Jikihara Hiroaki,
Morishige KenIchiro,
Miyake Akira,
Terakawa Naoki,
Tanizawa Osamu
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910540523
Subject(s) - transforming growth factor , autocrine signalling , tgf alpha , epidermal growth factor receptor , cancer research , transforming growth factor, beta 3 , growth factor , growth factor receptor inhibitor , mechanism (biology) , cell growth , endometrial cancer , cell culture , epidermal growth factor , receptor , medicine , biology , endocrinology , cancer , genetics , physics , quantum mechanics
Transforming growth factor‐β 1 (TGF‐β 1 ) enhanced cell proliferation in a concentration‐dependent manner in a human endometrial cancer cell line, IK‐90. Scatchard analysis of TGF‐β 1 receptor in IK‐90 cells, using 125 I‐TGF‐β 1 as a ligand, revealed the presence of a class of high‐affinity TGF‐β 1 receptors (2,000 sites per cell, K D = 74pM). Moreover, IK‐90 cells produced and secreted TGF‐β 1 : TGF‐β 1 messenger RNA was detected at 2.5 and 4.0 kb by Northern‐blot analysis using 32 P‐labeled TGF‐β 1 cDNA as a probe, and TGF‐β 1 activity in conditioned medium by the inhibition of 3 H‐thymidine uptake into CCI 64 mink lung epithelial cells. We investigated the regulation of TGF‐β 1 receptor by 4 kinds of growth factor: epidermal growth factor (EGF) but not TGF‐β 1 insulin or insulin‐like growth factor‐I increased the level of TGF‐β 1 binding sites in a concentration‐ and time‐dependent manner. These findings suggest that TGF‐β 1 may be a potential autocrine growth factor in a human endometrial cancer cell line IK‐90 and that this autocrine mechanism may be affected by EGF.