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Abnormalities in the p53 gene in tumors and cell lines of human squamous‐cell carcinomas of the head and neck
Author(s) -
Yin XiaoYing,
Smith Martin L.,
Whiteside Theresa L.,
Locker Joseph,
Johnson Jonas T.,
Herberman Ronald B.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910540226
Subject(s) - biology , gene , mutation , epidermoid carcinoma , mutant , cancer research , tumor suppressor gene , restriction fragment length polymorphism , allele , gene mutation , cell , microbiology and biotechnology , pathology , carcinoma , genetics , carcinogenesis , genotype , medicine
Abstract Abnormalities in the p53 gene were studied in a series of cell lines of human squamous‐cell carcinoma of the head and neck (SCCHN) and in tumor tissues. Restriction‐fragment‐length polymorphism (RFLP), quantitative hybridization and immunochemical analysis of mutant p53 proteins were combined to detect and characterize 3 different phases in the p53 gene alteration: mutation (in 9/9 cases), 17p 13 deletion (9/10 cases) and amplification of the non‐deleted allele (9/31 cases). In SCCHN, deletion of the p53 gene was nearly always accompanied by mutation, only one cell line studied having mutation without deletion. Alterations in the p53 gene are common in SCCHN, and involve a series of genetic events which occur in sequence during tumor progression.