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Detection of human papillomaviruses from histologically normal lymph nodes of Japanese cervical cancer patients by nested polymerase chain‐reaction assay
Author(s) -
Nawa Akihiro,
Kikkawa Fumitaka,
Kawai Michiyasu,
Mano Hisao,
Goto Setsuko,
Suganuma Nobuhiko,
Tomoda Yutaka,
Nishiyama Yukihiro,
Nakashima Nobuo
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530611
Subject(s) - lymph , lymph node , polymerase chain reaction , nested polymerase chain reaction , pathology , medicine , cervical cancer , radical hysterectomy , cancer , biology , gene , biochemistry
To determine the prognostic significance of human papilloma virus (HPV) DNA in histologically normal lymph nodes, we developed a nested polymerase chain‐reaction (PCR) method on HPV16, 18 and 33 DNAs for formalin‐fixed, paraffin‐embedded tissues. We investigated 370 histologically normal lymph nodes from 15 patients treated for stage‐IB/IIB (FIGO) invasive cervical cancer. HPV16 DNA was detected in 7 (47%) and HPV18 DNA in 3 (20%) of the cervical cancers. Examination of histologically normal lymph nodes from these 10 patients by nested PCR revealed HPV DNA in 5 (50%) of them; in all cases HPV type in lymph nodes and tumor was the same. Two of these 5 patients had a recurrence (pelvic cavity or lung) and died of cancer, although all 5 had had pelvic radiotherapy after radical hysterectomy and lymphadenectomy. These findings indicate that nested PCR is useful for evaluating early lymph‐node involvement retrospectively in HPV‐positive cases.