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Immortalization of human cervical keratinocytes by human papillomavirus type 33
Author(s) -
Gilles Christine,
Piette Jacques,
Rombouts Sonia,
Laurent Christian,
Foidart JeanMichel
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530527
Subject(s) - isochromosome , biology , cell culture , orfs , immortalised cell line , aneuploidy , population , neoplastic transformation , genetics , virology , microbiology and biotechnology , chromosome , karyotype , gene , carcinogenesis , open reading frame , medicine , environmental health , peptide sequence
Ten immortalized cell lines were established by transfection of human cervical keratinocytes (CK) with HPV‐33 DNA and some of their characteristics were investigated. The following observations were made: (a) several cell lines have reached over 100 population doublings in vitro ; (b) 3 transcripts were observed, 2 being encoded by the E6/E7 open reading frames (ORF s ); (c) cytogenetic analyses showed important genetic modifications such as aneuploidy and isochromosome formation of the q arm of chromosome 8; (d) 2 of the 10 cell lines developed colonies in soft agar but none was able to form tumors when injected S.C. into nude mice; (e) Southern analysis suggested that a single copy of HPV‐33 is integrated at a single common site within the genome of the 10 cell lines. These immortalized cell lines should be useful for studying mechanisms involved in proliferation, differentiation and neoplastic transformation of CK by HPV‐33.