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Binding activities of cis ‐platin‐damage‐recognition proteins in human tumour cell lines
Author(s) -
McLaughlin Karen,
Coren Grant,
Masters John,
Brown Robert
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530423
Subject(s) - cell culture , dna damage , oligonucleotide , biology , cell , dna , dna binding protein , microbiology and biotechnology , in vitro , cancer research , biochemistry , gene , genetics , transcription factor
Proteins can be detected by South‐western analyses of human tumour‐cell extracts binding to double‐stranded oligonucleotide DNA treated in vitro with the chemotherapeutic drug cis ‐diamminedichloroplatinum (11) (CDDP), but not to untreated DNA. The relative molecular masses of proteins binding to the CDDP‐treated double‐stranded oligonucleotide are 25, 48 and 97 kDa. The binding activity of 2 of the CDDP‐damage‐recognition proteins, of relative molecular mass 48 and 97 kDa, is greater in a CDDP‐resistant human ovarian tumour cell line than in the parental sensitive line. South‐western analysis of panel of human bladder cell lines and CDDP‐sensitive testicular cell lines show consistent patterns of CDDP‐damage‐recognition proteins within each cell type, however with differences between the 2 cell types. Binding of the proteins to CDDP‐damaged DNA and the altered binding activity detected in tumour cell lines suggests that alteration in damage‐recognition proteins could play a role in tumour response to CDDP.