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Detection of a progression‐linked DNA restriction fragment in rat hepatoma cells probed with a hexokinase CDNA
Author(s) -
Wigley W. Christian,
Corson Gary E.,
Nakashima Richard A.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530422
Subject(s) - biology , complementary dna , microbiology and biotechnology , restriction fragment , restriction enzyme , dna , restriction fragment length polymorphism , southern blot , genomic dna , gene , genetics , polymerase chain reaction
Abstract We have compared genomic DNA isolated from a series of rat hepatomas and from normal rat liver tissue by restriction fragment analysis. Our objective was to identify a tumor‐specific restriction fragment that would be present in rapidly growing tumors and missing in normal tissues. Using a cDNA probe specific for the C‐terminal half of rat brain hexokinase, we have identified several changes in DNA restriction fragment lengths in rat hepatoma vs. normal rat liver. Most of these involved restriction fragments that were present in normal rat DNA and missing in tumor DNA. However, one Hinfl fragment of M r = 13.4 kilobase pairs (kb) was found to be present in rapidly growing rat hepatomas, but missing in normal rat tissue and in hepatomas of slow or intermediate growth rate.