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Gp51 of bovine leukemia virus gene expression in hamster cells
Author(s) -
Slávikova Katarína,
Zajac Vladimir,
Klobuǐká Margita,
Aida Yoko,
Tevurková Viola
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530419
Subject(s) - biology , microbiology and biotechnology , epitope , virology , recombinant dna , gene , monoclonal antibody , transfection , virus , antibody , blot , genetics
Recombinant pMMEx‐bovine leukemia virus env gene DNA fragments were produced and expressed in eukaryotic cells. Clone C4, containing an Smal‐Smal fragment of the gene coding for gp51, was co‐transfected with pSVZneo DNA into Chinese hamster cells. About 800 geneticin‐resistant cell clones were isolated and then morphologically and biologically characterized. The presence of gp51 encoding env gene fragments was detected in 17 of them by Southern blotting. The expression of gp51 gene in hamster cells was confirmed by Western blotting of their lysates with monoclonal antibodies (MAbs) directed against different epitopes of gp51 of bovine leukemia virus. The immunoreactivity of the expressed peptides with MAbs directed against neutralizing epitopes of gp51 of bovine leukemia virus was confirmed.