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Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: Growth features and cytogenetics
Author(s) -
van den BergBakker Cornelia A. M.,
Hagemeijer Anne,
FrankenPostma Elsa M.,
Smit Vincent T. H. B. M.,
Kuppen Peter J. K.,
Claasen Hedda H. Van Ravenswaay,
Cornelisse Cees J.,
Schrier Peter I.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530415
Subject(s) - granulosa cell , karyotype , biology , cell culture , ovarian cancer , cytogenetics , clear cell carcinoma , ovary , serous carcinoma , carcinoma , cell , ovarian carcinoma , pathology , chromosome , cancer , genetics , medicine , gene
The characteristics of 7 newly established ovarian carcinoma cell lines and one granulosa tumor cell line obtained from tumor samples of 7 patients with varying histology of the primary tumor are reported. The cell lines were isolated from 5 serous carcinomas, a mucinous carcinoma, an endometrioid carcinoma and a granulosa cell tumor. All cell lines were passaged at least 25 times and showed stable growth rates. Colony‐forming efficiency varied on plastic from 2 to 57% and in agar from 0.01 to 9.30%. The DNA index of the granulosa tumor cell line was diploid, while the ovarian carcinoma cell lines were all aneuploid. In 2 cell lines polyploidisation occurred during culturing. A thorough cytogenetic analysis of 7 cell lines revealed that the granulosa tumor cell line has only minor cytogenetic abnormalities (+5, 22 q +). In contrast, the epithelial ovarian‐cancer cell lines gave very complex karyotypes with numerous markers and structurally rearranged chromosomes. The chromosomes most often in excess were 15 and 20. Structural rearrangements of chromosomes 1, 3, 7 and 11 were prominent in all ovarian cell lines. In addition, we found changes in chromosomes X, 5, 8 and 13 that have rarely been described before.