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Isolation and characterization of an oestrogen‐responsive breast‐cancer cell line, eff‐3
Author(s) -
Healicon R. M.,
Westley B. R.,
May F. E. B.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910530308
Subject(s) - cell culture , carcinoembryonic antigen , biology , receptor , cathepsin d , cancer research , endocrinology , medicine , intracellular , progesterone receptor , breast cancer , pathology , cancer , estrogen receptor , microbiology and biotechnology , genetics , biochemistry , enzyme
Abstract A novel oestrogen‐responsive breast‐tumour cell line, EFF‐3, has been established from a pleural exudate of a patient with metastatic breast cancer. The cells show morphological and immunohistochemical features consistent with their origin from a metastatic breast carcinoma. The cells aggregate and form sheets in culture, and electron microscopy confirms the presence of cell‐surface microvilli and intercellular tight junctions. The epithelial origin of EFF‐3 cells was confirmed by their expression of low‐molecular‐weight cytokeratins and carcinoembryonic antigen. The karyotype of the cells is markedly abnormal and there are large numbers of structurally abnormal chromosomes. EFF‐3 cells express oestrogen receptor, oestrogen‐receptor mRNA, their growth is oestrogen‐responsive, and specific genes are regulated by oestrogens. The pNR‐2/p52 and pNR‐25 oestrogen‐regulated mRNAs are induced 15‐ and 13‐ fold respectively by oestrogen, whereas the oestrogen‐receptor and cathepsin D mRNAs are not regulated. This pattern of regulation differs from that reported previously for other cell lines. The EFF‐3 cell line should be useful for studying the mechanisms involved in oestrogen‐stimulated proliferation and the factors determining the regulation of specific genes by oestrogens.