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Smoking‐related dna adducts in human gastric cancers
Author(s) -
Dyke G. W.,
Craven J. L.,
Hall R.,
Garner R. C.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910520602
Subject(s) - adduct , nucleotide , dna , cancer , dna adduct , epidemiology , medicine , biology , microbiology and biotechnology , chemistry , genetics , gene , organic chemistry
DNA was extracted from the tumour tissue of 26 patients (18 smokers, 8 non‐smokers) undergoing surgery for gastric cancer, and analyzed for the presence of DNA adducts by the 32 P‐post‐labelling method. Adducts were detected in all samples tested, and adduct levels ranged from 2 adducts/10 8 nucleotides to 60 adducts/10 8 nucleotides. In male subjects, adduct levels were significantly greater in the DNA of smokers than in that of non‐smokers. These results support epidemiological data suggesting that smoking is a risk factor for gastric cancer. © 1992 Wiley‐Liss, Inc.