Premium
PCR assay for chromosome 1p deletion in small neuroblastoma samples
Author(s) -
Peter Marline,
Michon Jean,
Vielh Philippe,
Neuenschwander Sylvia,
Nakamura Yusuke,
Sonsino Elise,
Zucker JeanMichel,
Vergnaud Gilles,
Thomas Gilles,
Delattre Olivier
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910520408
Subject(s) - neuroblastoma , loss of heterozygosity , ethidium bromide , microbiology and biotechnology , biology , chromosome , polymerase chain reaction , dna , genetics , allele , gene , cell culture
Abstract Deletion of the short arm of chromosome I is among the most recurrent cytogenetic alterations found in neuroblastoma and has been associated with short survival. However, this prognostic information, which relies on time‐consuming techniques, is not yet routinely exploited. In order to set up a reliable and simple routine test to determine Ip deletion in neuroblastoma, we have used the polymerase chain reaction to genotype neuroblastoma DNA at 2 loci containing a variable number of tandem repeats and located on the distal part of the short arm of chromosome I. Agarose gel electrophoresis and ethidium bromide staining of the amplification products enable a simple determination of constitutional and tumor genotypes at these loci to be made. A total of 37 samples from 29 patients were studied with this technique. Loss of heterozygosity (LOH) could be identified in 8 cases. In each case the results obtained were in agreement with those achieved by the Southern procedure. This technique will be of particular interest in the pretherapeutic analysis of Ip deletions in small tumor samples obtained by fine‐needle aspirates of the primary tumor. It should also enable retrospective studies from paraffin‐embedded tumor fragments to be made and provide information for the analysis of tumor heterogeneity in neuroblastoma and in other tumors with Ip deletions. © 1992 Wiley‐Liss, Inc.