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Human melanoma cell‐derived factor(S) stimulate fibroblast glycosaminoglycan synthesis
Author(s) -
Edward Michael,
Grant Allison W.,
Mackie Rona M.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910520327
Subject(s) - glycosaminoglycan , fibroblast , cell culture , glucosamine , hyaluronic acid , fibroblast growth factor , cell , melanoma , chondroitin , microbiology and biotechnology , chemistry , in vitro , basic fibroblast growth factor , biochemistry , biology , growth factor , cancer research , anatomy , genetics , receptor
Conditioned media from cultures of human metastatic Hs294T melanoma cells contain a factor/factors that promote(s) fibro‐blast‐mediated contraction of collagen lattices, and stimulate(s) fibroblast glycosaminoglycan (GAG) synthesis. Complete medium from melanoma cell cultures stimulated fibroblast hyaluronate synthesis 9.3‐fold, and sulphated GAG synthesis 2.6‐fold, as measured by 3 H‐glucosamine incorporation. 35 SO 4 incorporation into sulphated GAGS was essentially unaltered, the net result being a decrease in the degree of sulphation. Fibroblasts synthesized hyaluronate with an increased molecular weight when grown in the presence of the melanoma‐cell culture medium, while the molecular weights of heparan and chondroitin sulphates remained essentially unaltered. Our results indicate that the tumour‐cell‐derived factor(s) stimulate(s) changes in fibroblast glycosaminoglycan synthesis, and that these changes may facilitate tumour cell invasion in vivo . © 1992 Wiley‐Liss, Inc.