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Direct detection of Epstein‐Barr virus in peripheral blood and comparison of Epstein‐Barr virus genotypes present in direct specimens and lymphoblastoid cell lines established from nasopharyngeal carcinoma patients and healthy carriers in Hong Kong
Author(s) -
Lung Maria Li,
Lam Wai Pang,
Chan Kwok Hung,
Li Shaobing,
Sham Jonathan,
Choy Damon
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910520203
Subject(s) - virus , nasopharyngeal carcinoma , epstein–barr virus , throat , lymphoblast , virology , peripheral blood , biology , herpesviridae , pathology , biopsy , immunology , viral disease , medicine , cell culture , genetics , anatomy , radiation therapy
By means of a PCR assay, EBV was demonstrated directly in peripheral blood of previously infected individuals. The virus was detected in approximately 80% of specimens from EBV‐seropositive individuals, but not in cord‐blood lymphocytes by this method. When virus present in peripheral blood was compared to that observed directly in NPC biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (17%) and from that seen in throat washes in 1/22 cases (5%). The throat‐wash virus differed from the biopsy virus in 3/20 cases (15%). The prototype F virus was found in 7/10 LCLs (70%) established from NPC patients' peripheral blood, but was only detected in 2/9 specimens (22%) directly analyzed by the PCR assay. This finding suggests selective isolation of prototype F EBV in spontaneous LCLs established from NPC patients. © 1992 Wiley‐Liss, Inc.