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Effect of MHC class‐I transfection on local tumor growth and metastasis in an H‐2‐negative clone derived from a chemically induced fibrosarcoma
Author(s) -
Mialdea M. J.,
Martin J.,
Gaforio J. J.,
Algarra I.,
Pérez M.,
Gonzalez A.,
Ljunggren H. G.,
Garrido F.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910520127
Subject(s) - transfection , clone (java method) , fibrosarcoma , metastasis , biology , microbiology and biotechnology , cancer research , lewis lung carcinoma , ratón , gene , immunology , cancer , genetics
GR9 is a chemically induced fibrosarcoma composed of clones with different H‐2 class‐I expression. These clones differ with respect to local growth and spontaneous metastasis. The B9 clone (H‐2 negative) is highly tumorigenic (local growth) but of low metastatic potential (spontaneous metastasis assay). We have analyzed the effect that transfection of H‐2D d and H‐2K d genes on this clone have upon local growth, lung colonization after i.v. injection and ability to form spontaneous metastases. The results showed that the effect on local growth of transfection of the K d ‐gene was stronger than that of the D d gene. In addition, B9 co‐transfected with H‐2K d and D d genes showed the highest immunogenic properties in syngeneic BALB/c mice. Interestingly, the pSV 2 ‐neo transfected clone gave almost the same result as that obtained with D d transfection. Lung colonization after i.v. injection of the different clones (experimental metastasis), paralleled the results obtained for local growth: the number of lung nodules followed the cadence K d D d < K d < D d < pSV 2 . Survival of mice was always inversely correlated with local growth, e.g., all mice injected with 5 × 10 5 B9 H‐2K d D d transfected cells survived. In contrast, no mice injected with the B9 control did. These differences were abrogated in irradiated and nude BALB/c mice. Finally, all transfected clones remained non‐metastatic in a spontaneous metastasis assay, behaving as the control, non‐transfected B9 cells. © 1992 Wiley‐Liss, Inc.