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Insulin‐like growth‐factor‐binding protein gene expression and protein production by human tumour cell lines
Author(s) -
Reeve Julie G.,
Kirby Lemuel B.,
Brinkman Ad,
Hughes Stephanie A.,
Schwander Jurg,
Bleehen Norman M.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910510525
Subject(s) - biology , cell culture , microbiology and biotechnology , gene expression , insulin like growth factor binding protein , northern blot , insulin like growth factor 2 , cancer research , growth factor , cell growth , gene , insulin like growth factor , receptor , biochemistry , genetics
The secretion of insulin‐like growth‐factor‐binding proteins (IGFBPs) and expression of the genes encoding IGFBP‐I, IGFBP‐2 and IGFBP‐3 have been studied in a panel of cell lines derived from breast carcinomas, Wilms' tumour, neuroblastoma, retinoblastoma, colon carcinoma, liver adenocarcinoma, Burkitt's lymphoma and a non‐small‐cell lung carcinoma. All cell lines, with the exception of the Burkitt's lymphoma cell line, secreted IGFBPs, as detected by affinity labelling. A 34‐kDa BP was present in the conditioned media of all IGFBP‐secreting cell lines, whereas BPs ranging from 18 kDa to 53 kDa were variably secreted. All IGFBP‐secreting cell lines expressed the IGFBP‐2 gene as determined by Northern blot analysis. The Wilms' tumour, the neuroblastoma and the retinoblastoma cell line expressed the IGFBP‐2 gene only. All other cell lines, with the exception of the Burkitt's lymphoma, expressed the IGFBP‐2 gene and, in addition, either the IGFBP‐I gene and/or the IGFBP‐3 gene. IGFBP‐I gene expression could be detected by reverse transcriptase polymerase chain reaction only. IGFBP‐3 gene expression was detected by Northern blot analysis, but transcripts were less abundant than IGFBP‐2 mRNAs. These findings indicate that the expression of multiple BP genes and the secretion of BPs may be a common property of tumour cells. © 1992 Wifey‐Liss, Inc.