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A single human colonic adenoma cell line can be converted IN VITRO to both A colorectal adenocarcinoma and A mucinous carcinoma
Author(s) -
Paraskeva Christos,
Hague Angela,
Rooney Nicholas,
Williams Ann C.,
Harper Sara J.,
Hanlon Katherine A.,
Atkinson Rebecca J.,
Corfield Anthony P.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910510426
Subject(s) - adenocarcinoma , adenoma , karyotype , carcinogenesis , cancer research , carcinoma , pathology , malignant transformation , biology , neoplastic transformation , colorectal cancer , chromosome , cancer , medicine , genetics , gene
Abstract In a previous study, using a chemical carcinogen, we converted in vitro a non‐tumorigenic cell line derived from a human colorectal diploid adenoma, designated PC/AA, into a tumori‐genic cell line which, when inoculated into athymic nude mice, produced progressively growing adenocarcinomas. We now report that continuous in vitro passage of the PC/AA adenoma cell line resulted in its spontaneous transformation to a mucinous carcinoma with a modal karyotype of 51, XY, + i( Iq), +8, +9, +13, +i(13q), ‐21, +mar. These studies show that a single adenoma can be converted along 2 independent pathways, giving rise to either a mucinous carcinoma or an adenocarcinoma, and provide further experimental evidence for the adenoma‐carcinoma sequence. Cytogenetic changes which occur along both pathways to tumorigenicity include abnormalities of chromosome I and multiple copies of chromosome 13. These abnormalities may be important in tumour development and progression in colorectal carcinogenesis.