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Loss of HLA class‐I alleles, heavy chains and β 2 ‐microglobulin in colorectal cancer
Author(s) -
Kaklamanis Loukas,
Gatter Kevin C.,
Hill Ann B.,
Mortensen Neil,
Harris Adrian L.,
Krausa Peter,
McMichael Andrew,
Bodmer Julia G.,
Bodmer Walter F.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910510308
Subject(s) - beta 2 microglobulin , colorectal cancer , allele , human leukocyte antigen , genetics , biology , medicine , cancer research , cancer , immunology , antigen , gene
Using immunohistochemical methods, we have analysed colorectal biopsies of normal mucosa, metaplastic polyps (5 cases), adenomas (15 cases) and adenocarcinomas (70 cases) with 13 monoclonal antibodies (MAbs) to allelic products of the HLA‐A, B, C loci. Nine of the 70 carcinomas showed total loss of HLA Class‐I molecules due to an underlying defect regarding not only the expression of β 2 ‐microglobulin (·β 2 ‐m), but also the heavy chains of HLA A, B and C loci, or both. Much commoner was a loss of one or more Class‐I alleles as follows: Al/Aw36 (completely lost in 4 of 29 cases and focally lost in another 2), A2 (in 1 of 37 cases), A3 (in 2 of 14 cases), A11/28/31/33 (in 3 of 11 cases), B7 (in 3 of 13 and focally in 1 other case), B17 (in 1 case), Bw4 (in 8 of 45 and focally in another 6), Bw6 (in 9 of 62 and focally in another 3). Focal selective loss (Bw6 and a combined A 1 ‐Bw6), was observed in 2 adenomas. Normal colonic mucosa, as well as stromal and lymphoid cells present between the neoplastic glands, were studied in each case as a control. A particular allele was only considered to be lost by the malignant cells if it was still expressed on these adjacent tissues.