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ANti‐CD3 antibody‐treated mice: In Vivo induction of cytolytic activity and tnf production by lung leukocytes
Author(s) -
Stanňková Jana
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910510215
Subject(s) - spleen , tumor necrosis factor alpha , in vivo , cytolysis , cytokine , cd3 , antibody , biology , immunology , in vitro , microbiology and biotechnology , antigen , cd8 , cytotoxic t cell , biochemistry
Anti‐CD3 antibody induced non‐MHC‐restricted cytolytic activity in murine spleen cells within 4 hr of incubation in vitro and within 8 hr in vivo after intraperitoneal injection. Interstitial lung leukocytes acquired the capacity to kill both NK‐sensitive and ‐resistant targets within 24 hr after anti‐CD3 injection. In vivo ‐stimulated spleen cells produced significantly more IL‐2 and TNF than unstimulated cells. When these cells were restimulated with anti‐CD3 in vitro , a potentiation of TNF production was seen. Interstitial lung leukocytes doubled their TNF production ex vivo after anti‐CD3 injection, and the suppressed TNF production which was seen in lung cells from tumor‐bearing animals was restored after anti‐CD3 stimulation. The TNF production by alveolar macrophages was augmented 5‐fold 48 hr after injection of antibody. Anti‐CD3 antibody also induced significant accumulation of both TNFα and TNFβ mRNA in spleen and lung leukocytes. Pre‐treatment with anti‐TNF antibodies, both in vitro and in vivo , did not eliminate the cytolytic activation of lung and spleen cells. Our data indicate that anti‐CD3 antibodies can induce rapid activation of both cytolytic activity and cytokine production in lung lymphocytes and macrophages.