Premium
Differential expression of myogenic regulatory genes, Myo D1 and myogenin, in human rhabdomyosarcoma sublines
Author(s) -
Hosoi Hajime,
Sugimoto Tohru,
Hayashi Yasuhide,
Inaba Toshiya,
Horii Yoshihiro,
Morioka Hiroyuki,
Fushiki Shinji,
Hamazaki Minoru,
Sawada Tadashi
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910500626
Subject(s) - myogenin , biology , rhabdomyosarcoma , myod , desmin , microbiology and biotechnology , clone (java method) , myosin , pax3 , gene , vimentin , gene expression , sarcoma , genetics , pathology , immunohistochemistry , immunology , medicine , transcription factor
A cell line (SCMC‐MM‐1) was established from a human abdominal tumor that was initially diagnosed as a malignant mesenchymoma by histological, immunohistochemical and clinical criteria. The cell line was composed of 2 morphologically and immunohistochemically distinct cell types, one with a small polygonal phenotype (P‐type), characterized by the immune‐staining of vimentin and the presence of a few electron‐microscopically visible organelles, and the other with a giant tubular phenotype (T‐type), characterized by the immunostaining of desmin, α‐sarcomeric actin and skeletal‐muscle myosin, and the presence of thick and thin myofilaments and Z‐line materials. The parental cell line was cloned into 2 sublines, a P‐type clone (SCMC‐MM‐1‐19P) and a T‐type clone (SCMC‐MM‐1–1T), which shared both 2q37 and 11p15 translocations, the characteristic chromosomal aberrations for rhabdomyosar‐coma, with the parental SCMC‐MM‐I cell line. Northern‐blot analyses of the myogenic regulatory genes, including Myo DI and myogenin, demonstrated the expression of Myo DI in both of these sublines. Myogenin was very weakly expressed in the SCMC‐MM‐1–19P subline, but strongly expressed in the SCMC‐MM‐1–1T subline. Chromosomal and myogenic‐regulatory‐gene analyses revealed that both of these sublines were rhabdomyo‐sarcoma cell lines. Furthermore, the regulatory‐gene analyses indicated that these 2 sublines represented 2 distinct differentiation stages of myoblasts, and that Myo D1 and myogenin could serve as the lineage marker and the differentiation marker, respectively, of human rhabdomyosarcoma.