Premium
An autocrine mitogenic activity produced by a pleural human mesothelioma cell line
Author(s) -
Lauber Béatrice,
Leuthold Margrit,
Schmitter Doris,
CanoSantos Josefina,
Waibel Robert,
Stahel Rolf A.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910500621
Subject(s) - autocrine signalling , cell culture , biology , cell growth , microbiology and biotechnology , platelet derived growth factor receptor , cell division , cell , growth factor , biochemistry , genetics , receptor
The pleural human mesothelioma cell line ZL5 established in our laboratory exhibits an unusual phenotype with adherent and floating cells. ZL5 cells grow in a chemically defined medium (ACL3*) and can be maintained over 3 weeks in protein‐free basal medium alone (RPMI). Basal medium conditioned by ZL5 cells possesses a mitogenic activity with an autocrine effect, as measured by cell counting and by a 3 H‐thymidine incorporation assay. Moreover, the conditioned medium affects the DNA synthesis of a variety of other lung‐derived cells. The active principle of medium conditioned by ZL5 cells is not identical to the defined growth‐factors EGF, PDGF, and TGF‐β, known to stimulate the growth of normal human mesothelial cells: treatment with these factors does not mimic the effect of conditioned medium on ZL5 cells. Our observations suggest that the mesothelioma cell line ZL5 produces an unknown autocrine mitogen.