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Invasive and metastatic properties of MCF‐7 cells and ras H ‐transfected MCF‐7 cell lines
Author(s) -
Gelmann Edward P.,
Thompson Erik W.,
Sommers Connie L.
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910500431
Subject(s) - mcf 7 , transfection , cell culture , cancer research , cell , biology , microbiology and biotechnology , chemistry , oncology , medicine , genetics , cancer , cancer cell , human breast
In vitro invasion and in vivo metastasis assays were performed with a panel of MCF‐7 cells transfected with isogenic constructs of mutated ras H genes. Both increased levels of ras H expression and ras H oncogene activation increased activity of derivative cell lines in in vitro invasion assays. In vivo formation of spontaneous metastases was assessed after intradermal inoculation of MCF‐7 cells in the vicinity of the mammary fat pads of ovariectomized nude mice. No metastases were seen in the absence of estradiol treatment of the mice. With estradiol supplementation of the mice both the ras H ‐transfected and control transfected cell lines gave a higher incidence of metastases than parental MCF‐7 cells. Prolonged treatment of mice with exogenous estradiol (60 days vs. 21 days) resulted in more frequent metastases to liver and lung at the end of the 90‐day observation period. In contrast to activated ras H ‐gene enhancement of metastatic capacity of rodent fibroblast and epithelial cell lines, there was no correlation of ras H expression with in vivo metastatic capacity of a human mammary carcinoma cell line.