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Infrequent mutation of the ras genes in skin tumors of xeroderma pigmentosum patients in Japan
Author(s) -
Ishizaki Kanji,
Tsujimura Tohru,
Nakai Masanao,
Nishigori Chikako,
Sato Kenji,
Katayama Shoichi,
Kurimura Osamu,
Yoshikawa Kunihiko,
Imamura Sadao,
Ikenaga Mituo
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910500309
Subject(s) - xeroderma pigmentosum , gene , mutation , biology , oligonucleotide , photodermatosis , microbiology and biotechnology , genetics , cancer research , dna repair
By using PCR amplification and oligonucleotide mismatch hybridization, base‐substitution mutations of the ras genes in 26 skin tumors of Japanese xeroderma pigmentosum (XP) patients were studied. Thin sections of tumor tissues which were fixed and embedded in paraffin blocks were used in this study. After analyzing codons 12, 13 and 61 of the H‐, K‐ and N‐ ras genes by using 66 oligomer probes, we detected only one mutation of the K‐ ras gene at codon 61 in one tumor sample. All the other tumors were therefore considered not to have a mutation in the ras genes. These results suggest that mutations of the ras genes are not particularly associated with skin tumors of Japanese XP patients.