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Immunization against polyoma tumors with synthetic peptides derived from the sequences of middle‐and large‐T antigens
Author(s) -
ReinholdssonLjunggren Git,
Ramqvist Torbjörn,
ÄhrlundRichter Lars,
Dalianis Tina
Publication year - 1992
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910500128
Subject(s) - immunogenicity , epitope , antigen , biology , immunization , virology , peptide , microbiology and biotechnology , immunology , biochemistry
We have used 9 synthetic peptides corresponding to sequences of polyoma virus small‐T, middle‐T and large‐T antigens as immunogens in order to map antigenic epitopes that can induce polyoma‐tumor‐specific immunity in different mouse strains. We found that immunization of mice with synthetic peptides derived from amino acid (aa) sequences common to all 3 T‐antigens (aa 1–19), or sequences common to only middle‐T and small‐T (aa 162–176), as well as synthetic peptides unique for middle‐T (aa 269–282 and 371–381), or unique for large‐T (aa 108–124, 316–333 and 436–449) can induce immunity against polyoma tumors. The synthetic peptides can be divided into 3 types with regard to immunogenicity; (i) peptides that immunize in more than one mouse strain and may represent immunodominant sites, (ii) peptides that may be immunogenic in only one strain, and thus strain‐specific, and finally (iii) peptides that do not immunize in the strains tested so far.