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Differential mRNA levels of c‐ myc , c‐ fos and MHC class I in several clones of a murine fibrosarcoma
Author(s) -
Gaforio J. J.,
Pérez M.,
Algarra I.,
Mialdea M. J.,
Ljunggren H. G.,
Garrido F.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910490618
Subject(s) - fibrosarcoma , mhc class i , biology , messenger rna , microbiology and biotechnology , mhc restriction , transcription (linguistics) , major histocompatibility complex , clone (java method) , oncogene , c fos , gene expression , transporter associated with antigen processing , antigen , population , cancer research , cell , gene , immunology , genetics , cell cycle , medicine , linguistics , philosophy , environmental health
We have evaluated the relationship between MHC class‐I, c‐ myc and c‐ fos proto‐oncogene expression in several clones of a methylcholanthrene‐induced fibrosarcoma (GR9) which originated in a BALB/c mouse. These clones represent a heterogeneous population and differ markedly with regard to H‐2 class‐I cell‐surface expression, local tumor growth, NK sensitivity and metastatic potential. In the present study we show that cell‐surface expression of MHC class‐I antigens correlates inversely with levels of c‐ myc mRNA transcripts, On the other hand, mRNA levels of c‐ fos are correlated directly with the amount of mRNA of MHC class I. Treatment of the B9 clone with gamma interferon increased mRNA transcription and surface expression of H‐2 class‐I antigens, while c‐ myc transcription was simultaneously down‐regulated. In contrast, c‐ fos mRNA levels remained unaltered.