Premium
Expression of integrin receptors on 45 clinical neuroblastoma specimens
Author(s) -
Favrot Marie C.,
Combaret Valérie,
Goillot Evelyne,
Lutz Patrick,
Frappaz Didier,
Thiesse Philippe,
Thyss Antoine,
Dolbeau Dominique,
Bouffet Eric,
Tabone Eric,
Philip Thierry
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910490306
Subject(s) - neuroblastoma , pathology , biology , stage (stratigraphy) , integrin , receptor , microbiology and biotechnology , medicine , cell culture , genetics , paleontology
Immunohistological expression of integrins has been analyzed on 45 neuroblastoma specimens representative of the different clinical and histological forms of the tumor. None of the specimens expressed the α 5 chain of the integrins. The β 1 chain was expressed on all specimens, the α 1 chain on 44 specimens and the α 3 chain on 42; the 4 specimens which lacked α 1 or α 3 were stage‐4 neuroblastomas. The α 2 chain was expressed on 18 specimens, and the α 6 chain on 17;15 reacted with both. Their reactivity was related to the maturation of the tumor rather than the stage of the disease: they were expressed on lowgrade, well‐differentiated specimens; stage 3‐4 neuroblastoma specimens analyzed at diagnosis were negative, but usually expressed both chains when analyzed after in vivo differentiation by chemotherapy. α v reacted with 18 specimens and β 3 with 12, without strict relation with the stage of the disease and/or its degree of differentiation; 9 well‐differentiated specimens expressed the β 4 chain; only 4 well‐differentiated specimens expressed the α 4 chain. The 4 specimens which lacked α 1 ‐β 1 or α 3 ‐β 1 expression had n‐ myc amplification, whereas those which expressed either α 4 , β 4 , β 3 , or α v had no amplification. Furthermore, the expression of the 3 heterodimers α 4 ‐β 1 , α v ‐β 3 and α 6 ‐β 4 was essentially observed on primary tumors which developed in the mediastinum. The expression of α 2 ‐β 1 and α 6 ‐β 1 was observed on both n‐ myc ‐positive and ‐negative specimens. β 1 and α 3 , were diffusely expressed on all counterparts of these tumors, from undifferentiated neuroblasts to ganglion and Schwann cells. The α 1 chain reacted with undifferentiated and intermediate neuroblasts as well as with Schwann cells, but ganglion cells were negative. α 2 and α 6 chains were negative on undifferentiated neuroblasts, variably expressed on intermediate neuroblasts, and restricted to Schwann cells in ganglioneuroma. The expression of α 4 and β 4 was restricted to Schwann cells. α v and β 3 occasionally reacted with undifferentiated and intermediate neuroblasts; α v was strongly positive on Schwann cells but negative on ganglion cells, whereas β 3 was positive on both neuronal and non‐neuronal populations.