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Production and characterization of two immunotoxins specific for M5b ANLL leukaemia
Author(s) -
Tecce R.,
Fraioli R.,
De Fabritiis P.,
Sandrelli A.,
Savarese A.,
Santoro L.,
Cuomo M.,
Natali P. G.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910490228
Subject(s) - immunotoxin , cytotoxicity , bone marrow , cancer research , saporin , leukemia , biology , immunology , microbiology and biotechnology , in vitro , biochemistry
We describe the production and functional characterization of 2 monocytic‐cell‐lineage‐specific immunotoxins constructed with saporin emitoxin (SAP) from Saponaria officinalis . Interest in the production of these immunotoxins, of possible clinical relevance, has been raised by the availability of 2 MAbs of high specificity for circulating monocytes and M5b ANLL, thus envisaging their potential use in bone‐marrow purging. SAP emitoxin was selected on the basis of the low cytotoxicity in unconjugated form, as opposed to highly specific cytotoxicity and favourable pharmacokinetical properties in the conjugated form. SPDP conjugation produced immunotoxins which retained serological specificity and protein‐synthesis‐inhibitory activity. The 2 immunotoxins did not interfere with bone‐marrow progenitor‐cell growth in a CFU‐GM colony assay. On the contrary, they were capable of killing monocytic cells selectively, as demonstrated in phenotypical and functional assays. Thus these 2 novel immunotoxins appear to be promising reagents in purging autologous bone marrow prior to transplantation in patients suffering from monocytic leukaemia.