Premium
Comparison of neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts
Author(s) -
Ghim Shinje,
Christensen Neil D.,
Kreider John W.,
Bennett Jenson A.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910490224
Subject(s) - epitope , neutralization , bovine papillomavirus , virology , monoclonal antibody , capsid , fetus , infectivity , recombinant dna , antibody , microbiology and biotechnology , biology , fetal bovine serum , cell culture , virus , immunology , gene , pregnancy , biochemistry , genetics , genome
BPV‐I induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV‐I in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV‐1 or BPV‐2 virions, neutralize BPV‐1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV‐1 particles, serum of a rabbit immunized with denatured BPV‐1 particles, and sera from calves vaccinated with a recombinant L1 fusion protein did not neutralize BPV‐1 infection in either system. It was concluded that neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV‐1 capsid epitopes.