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Invasion by WC5 rat cerebellar cells is independent of RSV‐induced changes in growth and adhesion
Author(s) -
BradyKalnay Susann M.,
Boghaert Erwin R.,
Zimmer Stephen,
Soll David R.,
Brackenbury Robert
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910490217
Subject(s) - rous sarcoma virus , biology , cell culture , in vitro , adhesion , cell adhesion , glial fibrillary acidic protein , microbiology and biotechnology , cell , virus , chemistry , virology , biochemistry , immunology , immunohistochemistry , genetics , organic chemistry
The WC5 rat cerebellar cell line, which is infected with a Rous sarcoma virus that is temperature‐sensitive for pp60 src transformation, shows temperature‐dependent expression of the neural‐cell‐adhesion molecule (N‐CAM) and glial fibrillary acidic protein (GFAP). We found that WC5 cells maintained at the non‐permissive temperature in both monolayer cultures and spheroids are subject to density‐dependent inhibition of growth, whereas cells maintained at the permissive temperature continued to grow. The movement of isolated WC5 cells at both temperatures was similar, while the migration of WC5 cells out of 3‐dimensional aggregates was faster at the non‐permissive temperature. We tested whether the RSV‐induced changes affect the invasion of the WC5 cells in 2 in vitro assays: the chorio‐allantoic‐membrane assay and the chick‐heart‐fragment assay. In both assays, WC5 cells grown at either temperature were invasive. These results indicate that growth rate is unrelated to invasion and that loss of N‐CAM‐mediated cell‐cell adhesion is not necessary for invasion.