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Glutathione and cellular response of ACNU‐resistant rat glioma sublines to drugs and radiation
Author(s) -
Saito Yuji,
Hotta Takuhiro,
Mikami Takasi,
Kurisu Kaoru,
Yamada Kenji,
Kiya Katuzou,
Kawamoto Keiichi,
Uozumi Tohru,
Nakada Yosihiro
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910480612
Subject(s) - glutathione , radioresistance , intracellular , vinblastine , glioma , radiosensitivity , radiation sensitivity , cell culture , biology , pharmacology , microbiology and biotechnology , cancer research , chemistry , biochemistry , medicine , chemotherapy , radiation therapy , irradiation , genetics , enzyme , nuclear physics , physics
Abstract Three ACNU‐resistant sublines (R 1 , R 3 and R 12 ) from rat glioma 9L cells showed cross‐resistance to vinblastine, adriamycin, and VP‐16. Among these, the R 3 subline also acquired radioresistance under aerobic conditions. Total glutathione levels In these sublines were elevated 2‐to 3‐fold. Treatment of the cells with BSO, a specific inhibitor of GSH synthesis, resulted in decreased intracellular total glutathione levels in all 4 cell lines to about 10% of control levels. However, sensitivity to radiation or to chemicals did not change accordingly. Treatment of 9L cells with OTZ, a precursor of cystelne, resulted in a rise in intracellular GSH levels but it did not correlate with sensitivity to X‐ray or to ACNU. These results suggest that, in terms of cellular sensitivity to radiation or ACNU, total glutathione level alone cannot serve as a predictive indicator.