Premium
Protein kinase C activity as marker for colorectal cancer
Author(s) -
Sakanoue Youichirou,
Hatada Takuya,
Kusunoki Masato,
Yanagi Hidenori,
Yamamura Takehira,
Utsunomiya Joji
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910480602
Subject(s) - colorectal cancer , cancer research , protein kinase a , cancer , medicine , oncology , biology , kinase , genetics
Protein kinase C (PKC) activity was measured in normal‐appearing colonic mucosa from patients undergoing colonic resection. Comparisons were made between cases with and without colorectal cancers. PKC activity in cytosolic and particulate fractions was significantly reduced in patients with colorectal cancer: 40 ± 9 and 114 ± 29 (pmol/min/mg) respectively, compared with patients without cancer; 129 ± 11 and 250 ± 38 (pmol/min/mg) ( p < 0.05). Normal‐appearing colonic mucosa in patients with cancer showed significantly decreased total PKC activities in the cytosolic and particulate fractions compared with those In patients without cancer (10 ± I and 20 ± 3 vs. 30 ± 2 and 33 ± 6) (pmol/min/g tissue) ( p < 0.05). The cellular distribution (% particulate fraction) of PKC activity in normal‐appearing mucosa in patients with cancer (64%) was higher than in patients without cancer (49%) ( p < 0.05). Our data suggest that PKC activity may be used as a biological marker of risk of developing colorectal cancer or risk of bearing an asymptomatic tumor.