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A cell line (LIM 2463) derived from a tubulovillous adenoma of the rectum
Author(s) -
Whitehead Robert H.,
van Eeden Pauline,
Lukeis Robyn E.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910480511
Subject(s) - biology , clone (java method) , brush border , staining , rectum , mucus , antibody , microbiology and biotechnology , pathology , cancer research , immunology , medicine , genetics , dna , ecology , vesicle , membrane
We describe a new epithelial cell line (LIM 2463) derived from tubulovillous adenoma of the rectum. The cells grow as organoids and secrete large amounts of mucus. The cells are polarized, with a microvillar brush border, and express dipeptidyl peptidase IV in a polarized manner. No staining was seen with 3 other antibodies directed against other brush‐border hydrolases or disaccharidases. Focal polarized staining was obtained with 2 antibodies directed against other brush‐border‐associated peptides. The cells are aneuploid with a distinctive karyotype (48, XX, +9, +13). All attempts to clone the cells in semi‐solid agar or to grow them as xenografts in nude athymic mice have failed.