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Tumour‐growth suppression in nude mice by a murine monoclonal antibody: Factors hampering successful therapy
Author(s) -
Johansson Christina,
Segrén Sverker,
Lindholm Leif
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910480224
Subject(s) - antibody , monoclonal antibody , in vivo , in vitro , ratón , antigen , cancer research , immunology , biology , growth inhibition , biochemistry , microbiology and biotechnology
Abstract The murlne MAb C215 has been shown to mediate ADMMC In vitro and to have a tumour‐growth‐suppressive effect on xenografted COLO 205 human colocarcinoma cells in nude mice. To overcome the limitations of MAb therapy, it is necessary to understand the underlying mechanisms of tumour‐ growth suppression. In the present work, we have used C215 to define the Importance of different parameters involved in tumour therapy with murine IgG2a antibodies. The results show that there exists a period of roughly 2 days after inoculation into animals during which the tumour cells are sensitive to an inhibitory antibody‐mediated effect. After this initial period, the In‐vlvo sensitivity of tumour cells to antibody‐ mediated Inhibition Is much reduced. Tumour cells can remain “dormant” and, despite ongoing antibody treatment, develop Into tumours with a reduced growth rate, which is not caused by outgrowth of antigen‐deficient tumour cells. Finally, a pronounced dependence of antibody‐mediated tumour suppression on antibody dose was observed.