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HLA antigen expression in routinely processed head and neck squamous cell carcinoma primary lesions of different sites
Author(s) -
Mattijssen Vera,
de Mulder Pieter H. M.,
Schalkwijk Lia,
Manni Johannes J.,
HofGrootenboer Bep Van'T,
Ruiter Dirk J.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910470719
Subject(s) - human leukocyte antigen , pathology , immunohistochemistry , cd8 , monoclonal antibody , antigen , head and neck squamous cell carcinoma , carcinoma , antibody , medicine , cancer , head and neck cancer , immunology
HLA class I and II antigen expression in routinely processed head and neck squamous cell carcinoma (HNSCC) primary lesions was evaluated. Paraffin embedded samples from 66 squamous cell carcinomas and 7 verrucous carcinomas were studied immunohistochemically using recently developed anti‐HLA class I monoclonal antibodies (MAbs) HC10 and HCA2, and anti‐HLA‐DR rabbit serum. Percent stained tumor cells were scored in 1 of 5 categories. The scores of 40 tumors were compared to the staining results obtained on frozen sections of the corresponding lesions, including those of the anti‐class I MAb W6/32. High percentage‐matched scores for paraffin and frozen sections were obtained, with HC10 vs. HC10, HC10 vs. W6/32, and anti‐HLA‐DR vs. anti‐HLA‐DR showing the best correlations. In the squamous cell carcinomas HLA class I expression was high (i.e. , in 49/66 lesions more than 50% cells were stained), and correlated with the degree of differentiation, and inversely with the modified Jakobsson score. HLA class II expression (more than 5% cells stained) was found in 21/66 tumors and correlated inversely with the degree of differentiation. All verrucous carcinomas exhibited very high HLA class I expression, whereas class II was locally expressed in 5/7 lesions. Comparison of 4 subsites of HNSCC showed that carcinomas of the oral cavity had the highest HLA class I expression. This suggests susceptibility to CD8 + T cells, and together with the well developed submucosal lymphoid tissue, makes the oral cavity carcinomas probably well suited for local immunotherapeutic approaches in HNSCC.