Premium
HLA class‐II antigen‐mediated induction of a proliferative response to anti‐IgM in human B lymphocytes
Author(s) -
Mooney N.,
van Alewyk D.,
BrickGhannan C.,
Charron D.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910470709
Subject(s) - human leukocyte antigen , antigen , biology , monoclonal antibody , immunology , signal transduction , phosphatidylinositol , microbiology and biotechnology , antibody
Signal transduction via HLA class‐II antigens has been studied using human resting B lymphocytes and monoclonal anti‐HLA class‐II antibodies. An increased intracellular calcium flux, phosphatidylinositol biphosphate hydrolysis and activation of protein kinase C have all been observed following signal transduction via HLA‐class‐II molecules. The interaction of HLA‐class‐II‐mediated signalling with slg‐mediated signalling has been studied using a non‐mitogenic anti‐sIg. This combination provides a model for T‐cell‐dependent antigenic stimulation. The results demonstrate that stimulation via HLA class‐II antigens permits a proliferative response to an otherwise non‐mitogenic anti‐sIg and that this effect is not restricted to one HLA class‐II isotype. These data suggest that HLA‐class‐II‐mediated signalling is important in responses to T‐cell‐dependent antigens.