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A novel prostate carcinoma‐associated glycoprotein complex (PAC) recognized by monoclonal antibody turp‐27
Author(s) -
Wright George L.,
Beckett Mary Lou,
Lipford Grayson B.,
Haley Cara L.,
Schellhammer Paul F.
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910470516
Subject(s) - prostate , antigen , pathology , monoclonal antibody , biology , glycoprotein , prostate cancer , immunohistochemistry , prostate specific antigen , carcinoma , antibody , medicine , cancer , microbiology and biotechnology , immunology
A prostate carcinoma‐associated antigen recognized by MAb TURP‐27 was characterized immunohistochemically and biochemically. TURP‐27 antigen was found localized in the cell membrane and cytoplasm of the ductal epithelial cells of normal (10%), benign (75‐100%) and malignant (20‐100%) prostate cells. Fetal prostate tissues were also found to express the TURP‐27 antigen, suggesting expression early in development. This antigen was not expressed by non‐prostate tumors examined, but significant cross‐reactivity was observed in myelinated nerves while minor cross‐reactivity was seen in certain lymphocyte subsets, cells in the adrenal medulla and chief cells of stomach. Immunoblotting and biochemical data demonstrated that the TURP‐27 antigen is a sialic‐acid‐containing glycoprotein complex with major molecular species in prostate tissues of 310‐250, 180, 140, 115, 95‐90,69, and 40‐ to 35‐kDa. Immunoblotting patterns similar to those observed for prostate tissues were also seen in CNS extracts with the exception of the 69 and 40‐ to 35‐kDa proteins. This prostate carcinoma‐associated sialoglycoprotein complex (PAC) recognized by MAb TURP‐27 is likely to represent a novel tumor antigen expressed by prostate tumors.