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Characterization by somatic cell genetics of a monoclonal antibody to the MDR 1 gene product (P‐glycoprotein): Determination of p‐glycoprotein expression in multi‐drug‐resistant kb and cem cell variants
Author(s) -
Cenciarelli Cristina,
Currier Stephen J.,
Willingham Mark C.,
Thiebaut Franz,
Germann Ursula A.,
Rutherford Angelina V.,
Gottesman Michael M.,
Barca Stefano,
Tombesi Marina,
Morrone Stefania,
Santoni Angela,
Mariani Massimo,
Ramoni Carlo,
Dupuis Maria Luisa,
Cianfriglia Maurizio
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910470411
Subject(s) - monoclonal antibody , biology , epitope , microbiology and biotechnology , p glycoprotein , antibody , flow cytometry , glycoprotein , gene , gene product , gene expression , multiple drug resistance , drug resistance , immunology , genetics
We isolated an IgG 2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi‐drug‐resistant (MDR) human cells. Its specificity toward the MDR I gene product (P‐glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDR I gene in interspecific mouse × human cell hybrids, and the reactivity of several different MDR I gene‐expressing cells with Mab57, particularly insect cells acutely infected with a baculovirus encoding the MDR I gene. Mab57 can be used to detect, by flow cytometry, variations in the relative drug‐resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody‐dependent cell‐mediated (ADCC) assay system as well as in detecting P‐glycoprotein expression in normal and malignant tissues and cells.