Premium
Loss of Y chromosome with retention of Y heterochromatin in a marker chromosome from a human melanoma
Author(s) -
Doneda Luisa,
Larizza Lidia
Publication year - 1991
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910470127
Subject(s) - heterochromatin , biology , karyotype , chromosome , marker chromosome , y chromosome , genetics , monosomy , cytogenetics , microbiology and biotechnology , gene
A single copy of a der 15 chromosome (m3) characterized by a C‐ and distamycin A‐Dapi‐positive region was observed in the ‐Y hyperploid karyotype of a primary human melanoma (Me 1402). The heterochromatic region was located pericentromerically, adjacent at one end to the NOR region of chromosome 15, and at the other to an unclassifiable chromosomal piece. We established that the C‐positive block in the marker chromosome originated from Y heterochromatin by high‐stringency in situ hybridization with a DNA probe for the 2.1 Hae III Y‐specific repeat. Loss of the Y chromosome in tumors has been considered to be a secondary event associated with malignant evolution. It is significant that Me 1402 cells, which are highly malignant, lack the Y chromosome, but retain its heterochromatic portion in the rearranged m3 chromosome.