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Establishment and characterization of a new human B‐cell line (ONHL‐1) from non‐Hodgkin's lymphoma: Constant expression of bcl‐2 gene during mitogen‐induced growth inhibition
Author(s) -
Matsumura Itaru,
Tamaki Toshiharu,
Katagiri Shuichi,
Taniwaki Masafumi,
Tominaga Nobuhiko,
Oritani Kenji,
Iida Masato,
Yagura Hirosuke,
Yonezawa Takeshi,
Tarui Seiichiro
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460626
Subject(s) - biology , microbiology and biotechnology , cell culture , locus (genetics) , gene , cell growth , gene expression , gene rearrangement , lymphoma , genetics , immunology
A new B‐cell line (ONHL‐I) was established from non‐Hodgkin's lymphoma. ONHL‐I was free from Epstein‐Barr virus nuclear antigen and expressed CD20, CD24, and slg (μ, δ, γ and κ), thus being equivalent to the mature B‐cell stage. Chromosome analysis revealed a markedly abnormal pattern including 14q+ and 6q‐ In accordance with the positive expression of surface K light chains, one of the K genes was found to be rearranged. However, rearrangement of the À locus was also detected, contrary to the supposed hierarchy for the rearrangement of the light‐chain genes. The cell line showed rearrangement of the bcl‐2 gene. Further, the rearranged fragments of the J H , C A , and bci‐2 genes were of the same size in the EcoRI and Hind III digests on the same filter. This may suggest that the bcl‐2 gene is juxtaposed with the J H and C A locus. The proliferation of ONHL‐I was inhibited by adding Staphylococcus aureus Cowan I or 12‐O‐tetradecanoyl‐phorbol‐13‐acetate. During this growth inhibition, the expression of c‐ myc decreased, while that of bcl‐2 mRNA remained steady. This result suggests that not the bcl‐2 gene but other oncogenes, such as c‐ myc , play a key role in the proliferation of ONHL‐I. This agrees with the hypothesis that the bcl‐2 gene is not concerned with aggressive proliferation but with cell survival. This new cell line will therefore be of value in studying the differentiation and tumorigenesis of B cells.