Premium
A fluorouridine‐anti‐CEA immunoconjugate is therapeutically effective in a human colonic cancer xenograft model
Author(s) -
Shih Lisa B.,
Xuan Hong,
Sharkey Robert M.,
Goldenberg David M.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460625
Subject(s) - immunoconjugate , conjugate , monoclonal antibody , antibody , cancer research , immunotoxin , chemistry , radioimmunotherapy , antigen , flow cytometry , cytotoxic t cell , immunology , medicine , biochemistry , in vitro , mathematical analysis , mathematics
5‐fluorouridine (FUR), an antineoplastic agent, was sitespecifically conjugated to the carbohydrate moiety of a anticarcinoembryonic antigen (CEA) monoclonal antibody (MAb) by using amino‐dextran as the intermediate carrier. The final immunoconjugate contains approximately 30‐35 molecules of FUR per molecule of immunoglobulin, has immunoreactivity retained as examined by flow cytometry, and is cytotoxic to the target cells as examined by 75 selenomethionine incorporation studies. In the GW‐39/nude mouse model, the conjugate remained efficient in targeting the human colonic tumor and possessed greater inhibitory growth effects on the subcutaneous tumor than free FUR or an irrelevant antibody conjugate. In addition, the reduced host toxicity of the conjugate may permit the use of this agent in a high‐dose therapy of this tumor system.