Premium
Oncogene expression of fanft‐ or BBN‐induced rat urothelial cells
Author(s) -
DebiecRychter Maria,
Jones Richard F.,
Zukowski Kim,
Wang Ching Yung
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460527
Subject(s) - oncogene , carcinogenesis , immunohistochemistry , biology , nitrosamine , cancer research , microbiology and biotechnology , northern blot , gene product , cell culture , gene expression , gene , urothelial cell , pathology , carcinogen , bladder cancer , cell cycle , cancer , medicine , immunology , genetics
Proto‐oncogene expression by cultured urothelial cells prepared from the bladders of male F344 rats that had been treated with N ‐[4‐(5‐nitro‐2‐furyl)‐2‐thiazolyl]‐formamide (FANFT) or N ‐butyl‐ N ‐(4‐hydroxybutyl)nitrosamine (BBN) were examined. Although all of the cultured cells showed varying degrees of anchorage‐independent growth, only 9 of them were transplantable into nude mice. A Northern blot technique was employed for the detection of proto‐oncogene transcripts. The c‐Ha‐ ras transcripts were detected in all the cultured urothelial cells prepared from the carcinogentreated rats and in normal urothelial cells. However, the transcript levels were several‐fold higlher in the former than in normal cells. Increased expression of p21, as determined by immunohistochemical techniques, was also observed in all the original bladder tissues from which the cultures were derived. c‐ myc transcripts were detected in the cells from carcinogentreated rats but not in the normal cells. The presence of myc product in hyperplastic urothelial lesions and carcinomas of original bladder tissues was confirrned by immunohistochemical methods. Transcripts of mos, erb B, Ki‐ ras, abl and src were not detected. Since increased expression of c‐ myc and c‐Ha‐ ras were present in both transplantable and non‐transplantable cell lines, and the expression of p21 occurs in preneoplastic cells, this suggests that elevated expression of these 2 genes may be an early genetic event during bladder carcinogenesis in the rat and further alteration of these 2 genes or mutation of additional genes may be required for the completion of malignant transformation.