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Suppressive effects of intracellular glutathione on hydroxyl radical production induced by tumor necrosis factor
Author(s) -
Yamauchi Naofumi,
Watanabe Naoki,
Kuriyama Hiroshi,
Neda Hiroshi,
Maeda Masahiro,
Himeno Takeshi,
Tsuji Yasushi,
Nutsu Yoshiro
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460522
Subject(s) - glutathione , buthionine sulfoximine , intracellular , tumor necrosis factor alpha , cytotoxicity , hela , chemistry , hydroxyl radical , necrosis , biochemistry , cell culture , in vitro , pharmacology , biology , immunology , medicine , radical , enzyme , genetics
Abstract Protective effects of intracellular glutathione (GSH) against the cytotoxicity of human recombinant tumor necrosis factor (TNF) were investigated. Three tumor cell lines (L‐M, B‐16, HeLa) were used as target cells. Exposure of these cells to buthionine sulfoximine (BSO) or diethyl maleate (DEM) resulted in the depletion of intracellular GSH content to 5.2‐43.0% of control values and enhancement of their susceptibility to TNF cytotoxicity. The hydroxyl radical production in L‐M cells stimulated by TNF was increased by treatment with BSO or DEM. These results are consistent with the suggestion that intracellular GSH exerts its protective function against the cytocidal effect of TNF by inhibiting the hydroxyl radical production stimulated by TNF.