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Expression of PDGF β‐receptors in human meningioma cells
Author(s) -
Wang JiaLun,
Nistér Monica,
Hermansson Monica,
Westermark Bengt,
Pontén Jan
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460504
Subject(s) - platelet derived growth factor receptor , receptor , meningioma , biology , platelet derived growth factor , immunohistochemistry , microbiology and biotechnology , cell culture , in situ hybridization , pathology , growth factor , messenger rna , biochemistry , immunology , medicine , gene , genetics
Meningioma is a generally benign tumor derived from arachnoid tissue. We have investigated the presence of functionally active PDGF‐receptors on human meningioma cells in culture. Tumor samples were obtained from 3 surgically removed benign meningiomas and normal arachnoid tissue from an autopsy case. Binding studies were performed by using 125 I‐labelled recombinant PDGF‐AA and PDGF‐BB. Only 125 I‐PDGF‐BB showed specific binding to all tumor‐cell cultures after incubation of cells for 2 hr at 4°C. Effects of PDGF‐AA and PDGF‐BB on DNA synthesis were measured as 3 H‐thymidine incorporation during 48 hr of labelling cells maintained in Eagle's minimum essential medium 0.5% fetal calf serum. PDGF‐BB but not PDGF‐AA stimulated DNA synthesis in all 3 tumor‐cell cultures. Total cellular RNA was analyzed by Northern blotting and hybridization with a 32 P‐labelled human PDGF β‐receptor probe, and PDGF β‐receptor mRNA was found in both tumor and arachnoid cell cultures. Furthermore, PDGF β‐receptor mRNA was shown to be present in 2 meningioma biopsies and immunohistochemical staining revealed that PDGF β‐receptors are present in meningioma and arachnoid tissues in vivo. It appears that a possible way of maintaining human meningioma cell growth in vivo is through activation of PDGF β‐receptors.