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High frequency of Epstein‐Barr virus genome detection in Hodgkin's disease of HIV‐positive patients
Author(s) -
Uccini Stefania,
Monardo Francesca,
Stoppacciaro Antonella,
Gradilone Angela,
Aglianò Anna M.,
Faggioni Alberto,
Manzari Vittorio,
Vago Luca,
Cosianzi Giulio,
Ruco Luigi P.,
Baroni Carlo D.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460405
Subject(s) - southern blot , virus , epstein–barr virus , in situ hybridization , antigen , genome , virology , lymph , disease , lymphoma , reed–sternberg cell , gammaherpesvirinae , biology , herpesviridae , immunology , viral disease , medicine , pathology , gene , gene expression , genetics , hodgkin lymphoma
Lymph nodes obtained from 7 HIV‐positive and 20 HIV‐negative patients with Hodgkin's disease were examined for the presence of Epstein‐Barr virus antigens and genome. EBV antigens were observed in only 2 out of 20 HIV‐negative patients, whereas lymph nodes of HIV‐positive patients did not reveal evidence of EBV antigens. By In situ hybridization and Southern blot analysis, EBV genome was found in 5 out of 7 HIV‐positive patients; the EBV genome was detected in the nucleus of Reed‐Sternberg and Hodgkin's cells. EBV DNA was observed by in situ hybridization and Southern blot analysis in only out of 20 HIV‐negative patients with Hodgkin's disease. In both groups, Reed‐Sternberg and Hodgkin's cells were neg ative for C3d EBV receptor. Our results show a statistically significant increased expression of EBV DNA in HIV‐positive patients with Hodgkin's disease, as compared with HIV‐negative patients with HD.