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The use of clonogenic assays in assessing the response of human lung cancer cell lines to α and γ interferons alone or in combination with adriamycin
Author(s) -
Jabbar S. A. B.,
Twentyman P. R.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460335
Subject(s) - clonogenic assay , cell culture , cytotoxic t cell , biology , cancer research , immunology , cell , cell counting , microbiology and biotechnology , in vitro , cell cycle , biochemistry , genetics
The antiproliferative and cytotoxic effects of purified IFN‐α and recombinant IFN‐γ were investigated using both direct cell counting and a clonogenic assay on a panel of 5 established human lung cancer cell lines and for 2 of them also on their multidrug‐resistant counterparts. There was considerable heterogeneity in the response of the cell lines to the IFNs in terms of growth inhibition. Clonogenic assay of IFN‐treated cells indicated that, where a cell line had responded markedly to an IFN, only a small fraction of the cells remaining after IFN treatment were clonogenically viable. When cells were placed into the clonogenic assay in the presence of IFNs, the time course of colony formation was different from that seen in the control cultures for most of the cell lines. The measured “surviving fraction” was greatly dependent upon the time of colony counting. When the effects of IFNs in combination with ADM were studied, conclusions regarding the interaction of the effects of the agents also depended upon the time at which colonies were counted.