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K‐ ras mutations (codon 12) are not involved in down‐regulation of mhc class‐i genes in colon carcinomas
Author(s) -
Oliva Maria Rosario,
Cabrera Teresa,
Esquivias Javier,
PerezAyala Millán,
Redondo Maximino,
RuizCabello Francisco,
Garrido Federico
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460317
Subject(s) - point mutation , biology , gene , mutation , human leukocyte antigen , polymerase chain reaction , microbiology and biotechnology , genetics , major histocompatibility complex , antigen
Fifty‐eight colorectal carcinomas were studied for HLA class‐l antigen expression and for the presence of point mutations in codons 12 and 61 of the K‐ras gene. Eight carcinomas were completely negative for class I by the APAAP technique. Analyses using the polymerase chain reaction (PCR) method, together with selective hybridization using mutation‐specific synthetic oligonucleotides, demonstrated K‐ ras mutations in 14 cases (24.1 %), all of them in codon 12. None of the mutations corresponded to the negative cases for class‐l HLA antigen expression. We did not observe any correlation between K‐ ras mutations and the extent of tumor differentiation.