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Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease
Author(s) -
Abelev G. I.,
Karamova E. R.,
Bragina E. G.,
Andreeva N. E.,
Kruglova G. V.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460302
Subject(s) - waldenstrom macroglobulinemia , macroglobulinemia , chronic lymphocytic leukemia , bence jones protein , isotachophoresis , lymphoma , paraproteins , minimal residual disease , medicine , multiple myeloma , urine , pathology , monoclonal , immunoglobulin light chain , monoclonal antibody , antibody , leukemia , bone marrow , chemistry , immunology , electrode , electrolyte
We suggest that countercurrent isotachophoresis performed on cellulose acetate membranes (ITP‐CAM) should be used for detecting trace amounts of Bence‐Jones protein (BJP) in urine of patients with chronic lymphocytic leukemia (CLL), non‐Hodgkin's lymphoma (NHL) and related diseases. ITPCAM allows simultaneous concentration and electrophoretic separation of proteins present in highly diluted solutions, as well as easy immunological detection of separated substances. BJP was found in 24 out of 42 patients with CLL, 33 of 56 with NHL and 3 of 3 with Waldenstrom macroglobulinemia. Twenty‐three patients were followed during the course of chemo‐ or radiotherapy. In 19 cases the BJP findings correlated well with clinical status. In no case of partial or complete clinical response did BJP completely disappear from the urine.