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Human T‐cell leukemia virus type‐I‐infected T‐cell lines scarcely produce p56 lck , whether or not they express lck MRNA
Author(s) -
Ohhori Nobuhira,
Koga Yasuhiro,
Yoshida Hiroki,
Morita Minoru,
Kimura Genki,
Nomoto Kikuo
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460229
Subject(s) - t cell leukemia , messenger rna , biology , microbiology and biotechnology , cell culture , leukemia , t cell , gene , cd28 , human t lymphotropic virus 1 , virus , virology , gene expression , gene product , murine leukemia virus , immunology , genetics , immune system
We have previously reported that lck mRNA (a lymphocyte‐specific protein tyrosine kinase gene) is absent in human T‐cell leukemia virus type I (HTLV‐I)‐infected interleukin‐2(IL‐2)‐independent T‐cell lines, while HTLV‐I‐negative T‐cell lines and HTLV‐I‐positive IL‐2‐dependent ones express a large amount of lck mRNA. To further investigate the levels of lck expression, we prepared rabbit anti‐Lck antiserum directed against the synthetic oligopeptide of 32 amino acids corresponding to the carboxy terminus of this gene product, p56 lck . Using this antiserum, we show that HTLV‐I‐positive T‐cell lines, whether they are IL‐2‐dependent or not, scarcely express p56 lck . In other words, IL‐2‐dependent HTLV‐I‐positive T‐cell lines seldom produce p56 lck In spite of high expression of lck mRNA. Absence of p56 lck is suspected of playing an important role in malignant transformation of HTLV‐I‐infected T‐cells.