Premium
Baboon anti‐idiotype antibodies mimic a carcinoembryonic antigen epitope
Author(s) -
Losman Michele J.,
Monestier Marc,
Hansen Hans J.,
Goldenberg David M.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460228
Subject(s) - baboon , epitope , idiotype , carcinoembryonic antigen , antibody , monoclonal antibody , antigen , microbiology and biotechnology , chemistry , biology , immunology , virology , cancer , endocrinology , genetics
A baboon was immunized with NP‐4, a murine monoclonal antibody (MAb) to carcinoembryonic antigen (CEA). Antiidiotype antibodies were purified from the baboon serum by affinity chromatography on a NP‐4‐coupled matrix, followed by adsorption of the non‐specific antibodies on an irrelevant MAb. Baboon anti‐idiotype antibodies inhibited specifically the binding between NP‐4 and CEA. Mice immunized with baboon anti‐idiotype antibodies produced antibodies to the CEA epitope recognized by NP‐4. These results indicate that baboon anti‐idiotype antibodies functionally mimic a CEA epitope and that they can be suitable for idiotype therapy of human CEA‐producing carcinomas.