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Fusion of rous‐sarcoma‐virus‐transformed rat cells to morphologically normal human or rat cells results in transcriptional suppression of the provirus that depends on its chromosomal integration site
Author(s) -
Green A. R.,
Poole C. J.,
Povey S. M.,
Rowe D.,
Searle S.,
Wyke J. A.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460213
Subject(s) - rous sarcoma virus , provirus , biology , cell fusion , clone (java method) , virus , virology , long terminal repeat , genetics , cell , microbiology and biotechnology , gene , genome
The fusion of a Rous sarcoma virus (RSV)‐transformed rat fibroblast clone to at least 2 different human cell types reproducibly produces phenotypically normal hybrids. Analysis of such hybrids reveals that proviral silence is the result of transcriptional down‐regulation, presumably by a trans ‐acting human molecule. Furthermore, this phenomenon seems to be strongly influenced by the proviral chromosomal integration site and its imposition may entail a mechanism that is required only transiently.