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Identification of a 43‐kda nuclear antigen associated with proliferation by monoclonal antibody k 112
Author(s) -
Quak Jasper J.,
Dongen Guus Van,
Koken Marie A. E.,
Brakkee Joost G. P.,
Scheper Rik J.,
Balm Alfons J. M.,
Jong Luitzen De,
Snow Gordon B.,
Meijer Chris J. L. M.
Publication year - 1990
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910460111
Subject(s) - monoclonal antibody , antigen , nucleolus , mitosis , biology , microbiology and biotechnology , antibody , cell nucleus , cytoplasm , nuclear protein , proliferating cell nuclear antigen , cell , immunohistochemistry , immunology , genetics , gene , transcription factor
Abstract Monoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous‐cell carcinoma. The antibody detects a 43‐kDa nuclear antigen, with a pl of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell‐cycle‐related nuclear antigen molecules.